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| The one to identify with | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| PowerPlex®
16 System amplifies all 13 CODIS STR loci at once. |
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| Amplify DNA once, not twice. You no longer have to amplify each database or casework sample twice. With the PowerPlex® 16 System you can amplify all 13 CODIS STR loci at one time. No more extra set up time or run time for a second analysis. You spend less time looking at data and printing results. Plus, the PowerPlex® 16 System has been designed for use with the ABI PRISM® 310 Genetic Analyzer, the ABI PRISM® 377 DNA Sequencer, and the ABI PRISM® 3100 Genetic Analyzer. The STR loci amplified at ONE TIME with the PowerPlex® 16 System are: |
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| Get the sensitivity you require. The PowerPlex® 16 System provides maximum sensitivity with low quantities of DNA– it is optimized for use with 1ng of DNA. However, each lot is performance tested to provide reproducible results with 0.5ng of DNA. Additional studies show interpretable results can be obtained with <0.5ng of DNA. Find the validation you expect. Concordance PowerPlex® 16 is concordant with Applied Biosystem's ProfilerPlus™ and COfiler™ PCR Amplification Kits. Validation Studies. Promega's developmental validation was completed in October 2000. In 2001, more than 20 laboratories participated in a casework validation study to comply with SWGDAM guidelines. Over 2000 samples were typed by multiple CODIS laboratories to complete the required CODIS database validation. Results of these studies are published and are available upon request. Realize the speed and simplicity you want. In addition to the speed of one amplification, PowerPlex® 16 has four key features that make the system rapid and simple to use. Primer Set Consistent balance across loci and between dyes simplifies analysis of low amounts of template DNA and minor contributions of other DNA samples. Internal Lane Standard 600 This CXR-labeled marker offers the greatest precision available for DNA typing. Peaks range in size from 60-600bp. Fragments that are multiples of 100 bases are twice the height of other fragments to simplify size assignment. Allelic Ladders Allelic Ladders for all 16 loci are provided to simplify interpretation and improve software compatibility. PowerTyper™ Macros The PowerTyper™ Macros allow automatic assignment of genotypes using Genotyper® software. Receive the technical support you need. A national research survey cited the high quality of technical support provided by Promega as a major factor in customer satisfaction. Promega's Regional Technical Specialists and Technical Services Representatives are highly trained scientists who provide exceptional support for all genetic identity products. Promega also provides technical training and support for its global distribution network, conducts workshops in human DNA typing, and sponsors symposia on human identification. |
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| A
single 0.5ng genomic DNA sample was amplified using the PowerPlex®
16 System and detected using the ABI PRISM® 310 Genetic Analyzer.
The top panel displays the loci D3S1358, TH01, D21S11, D18S51 and Penta
E labeled with fluorescein. The second panel displays the loci D5S818,
D13S317,D7S820, D16S539, CSF1PO and Penta D labeled with JOE. The third
panel displays the loci Amelogenin, vWA, D8S1179, TPOX, and FGA labeled
with TMR. The last panel displays all 16 loci and the Internal Lane Standard
600 (ILS600). |
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| THE ILS600 Fragments are evenly spaced to assure optimum performance in size determination throughout the entire range. The fragments, that are multiples of 100 bases generate peaks approximately twice as high as those of the other fragments, simplifying visual identification. Each fragment contains the entire sequence of each smaller fragment, minimizing sizing inconsistency due to sequence variation observed with other available size markers. |
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| PowerPlex® 16 is ready to use. No preparation of primers or buffers is required. All components have been quality tested. Each PowerPlex® 16 System contains the appropriate Primer Pair Mix, Allelic Ladder Mix, 9947A Control DNA, and sufficient reagents for 100 or 400 reactions of 25µl each. |
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| PowerPlex® 16 System Population Statistics | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| Population statistics have been published in Levadokou, E. et al. (2001) J. Forensic Sci. 46(3), 736-61 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
