Specialty Application Areas

Human Identity Testing

GenePrint® Silver STR Systems

1 x 1ml
1 x 250µl
1 x 3µg
1 x 3µg
1 x 300µl
1 x 150µl

Description: The GenePrint® Silver STR Systems (a,b,c) provide a rapid, non-radioactive method, wich can be used to evaluate very small amounts (e.g., 1ng) of human DNA. The systems provide all of the materials required to amplify STR regions of purified genomic DNA except for Taq DNA polymerase and sample DNA. The amplified STR fragments are then separated by polyacrylamide gel electrophoresis and detected by silver staining.

The combination of SilverSTR®III, CTT and FFv provides access to seven of the thirteen core loci that comprise the Combined DNA Index System (CODIS) convicted offender database.

Features

• Economical: The GenePrint® Silver STR Systems do not require fluorescent detection equipment for analysis. The systems are ideal for labs that are starting STR analysis or do not wish to purchase expensive fluorescent detection equipment.

• Efficient: Sample-to-data analysis requires less than one day. Each multiplex allows simultaneous amplification of three non-overlapping STR loci for high discrimination power.

Description: The GenePrint® Fluorescent STR Systems have been developed for use with the Hitachi FMBIO® Fluorescence Imaging Systems and the ABI PRISM® 377 DNA Sequencer. One primer for each locus in a multiplex system has been labeled with fluorescein to allow fluorescent detection. Fluorescein has an excitation maximum at 488nm and a light emission maximum at 532nm. Therefore, the systems are also compatible with a variety of fluorescence detection instruments including the Molecular Dynamics FluorImager® and GenomyxSC™ Fluorescent Scanners, the ABI PRISM® 373 DNA Sequencer, the ABI PRISM® 310 Genetic Analyzer, and the Pharmacia ALF® (but not the ALFexpress®) DNA Sequencer.

Each System provides all the materials required to amplify STR regions of purified genomic DNA except Taq DNA polymerase. Amplification of sample DNA using the system components plus Taq DNA polymerase produces fluorescein-labeled fragments representing alleles from the template DNA. For instruments that support two-color fluorescent detection, additional precision may be achieved by inclusion of the Fluorescent Ladder (CXR), 60-400 Bases (Cat.#DG6221) in each gel lane.

Features

• High-Throughput Analysis: Analysis is achieved by comparison of amplified sample DNA fragments directly with the allelic ladder present for each locus.
• Efficient: The Fluorescent STR Multiplex Systems support simultaneous single-tube amplification of four polymorphic STR loci with non-overlapping allele size ranges.
• Allelic Ladders: Comparison of amplified alleles with the allelic ladders provided with each system allows rapid and reliable determination of allele content.

Description: The Fluorescent Monoplex STR Systems have been developed for use with the Hitachi FMBIO® Fluorescent Scanner, the FMBIO® II Fluorescent Scanner, and the Applied Biosystems ABI PRISM® 377 DNA Sequencer. One primer for each locus in a multiplex or monoplex system has been labeled with fluorescein to allow fluorescent detection. Fluorescein has an excitation maximum at 488nm and a light emission maximum at 532nm. Therefore, the systems are also compatible with a variety of fluorescence detection instruments including the Molecular Dynamics FluorImager® and GenomyxSC™ Fluorescent Scanners, the ABI PRISM® 373 DNA Sequencer, the ABI PRISM® 310 Genetic Analyzer, and the Pharmacia ALF® (but not the ALFexpress™) DNA Sequencer.

Each system provides all the materials required to amplify STR regions of purified genomic DNA except Taq DNA polymerase. Amplification of sample DNA using the system components plus Taq DNA polymerase produces fluorescein-labeled fragments representing alleles from the template DNA. For instruments that support two-color fluorescent detection, additional precision may be achieved by inclusion of the Fluorescent Ladder (CXR), 60-400 Bases (Cat. #DG6221) in each gel lane.

Features

• High-Throughput Analysis: Analysis is achieved by comparison of amplified sample DNA fragments directly with the allelic ladder present for each locus.
• Allelic Ladders: Comparison of amplified alleles with the allelic ladders provided with each system allows rapid and reliable determination of allele content.