:: POWER PLEX 16 HS SYSTEM ::
     
 
 
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Power Plex 16® HS

 
Description

The PowerPlex® 16 HS System(a–e) is a multiplex STR system for use in DNA typing. This system co-amplifies the loci D18S51, D21S11, TH01, D3S1358, Penta E (all labeled with fluorescein); FGA, TPOX, D8S1179, vWA and Amelogenin (all labeled with TMR); CSF1PO, D16S539, D7S820, D13S317, D5S818 and Penta D (all labeled with JOE). This multiplex includes all 13 CODIS STR markers, Amelogenin for gender determination and two, low-stutter, highly discriminating pentanucleotide STR markers. All sixteen loci are amplified simultaneously in a single tube and analyzed in a single injection. The PowerPlex® 16 HS System is compatible with the ABI PRISM® 310, 3100 and 3100-Avant Genetic Analyzers and the Applied Biosystems 3130 and 3130xl Genetic Analyzers.

         
Features
       

Robust: The PowerPlex® 16 HS System is more tolerant of PCR inhibitors than competing STR systems and the previous version of PowerPlex® 16. Generate profiles with samples that previously failed to amplify. Avoid costly and time-consuming sample cleanup.

Sensitive: Each lot is quality tested to produce full profiles from 100pg of DNA. Gain confidence in analysis of limited samples.

High Discrimination: The loci included in PowerPlex® 16 HS are more discriminating than competitive systems (1). Ideal for resolving partial matches or challenging familial cases.

Proven Design: Primer sequences, dyes and ladders are all unchanged from PowerPlex® 16. Expect concordance with existing databases.

Complete System: PowerPlex® 16 HS includes size standard, amplification-grade water and Taq DNA polymerase already in the master mix. Simple to order, easy to use.

         
Applications
 
Storage Conditions
  • Forensic DNA analysis.
    Human identity testing.
  • Paternity testing.
    Tissue culture strain identification.
Store at –20°C.
     
 
 

Figures

Sensitivity of the PowerPlex® 16 HS System. Decreasing amounts of template were amplified using the PowerPlex® 16 HS System. Each amplification was performed as directed in the PowerPlex® 16 HS System protocol. Once amplified, samples were separated on an Applied Biosystems 3130 Genetic Analyzer using 3kV, 5-second injection. In each case all expected alleles were called using a 50RFU threshold.

   
Product
Cat. #
Size
PowerPlex® 16 HS System
DC2101
100 reactions
DC2100
400 reactions
   
 
Available Separately
9947A DNA
DD1001
250ng
PowerPlex® Matrix Standards, 310
DG4640
1 each
PowerPlex® Matrix Standards, 3100/3130
DG4650
1 each
Water, Amplification Grade
DW0991
6,250μl (5 × 1,250μl)
Cat.# DW0991 For Laboratory Use. All other Cat.# Not for Medical Diagnostic Use.
 
 

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