Power Plex S5

For difficult samples.

STR (short tandem repeat) loci consist of short, repetitive sequence elements 3–7 base pairs in length (1–4). These repeats are well distributed throughout the human genome and are a rich source of highly polymorphic markers, which may be detected using the polymerase chain reaction (5–8). Alleles of STR loci are differentiated by the number of copies of the repeat sequence contained within the amplified region andare distinguished from one another using radioactive, silver stain or fluorescence detection following electrophoretic separation.

The PowerPlex® S5 System(a–c) allows co-amplification and detection of five loci (four STR loci and Amelogenin), including D8S1179, D18S51, Amelogenin, FGA and TH01.

One primer specific for each of Amelogenin, D18S51 and D8S1179 loci is labeled with fluorescein (FL) and one primer specific for each of the TH01 and FGA loci is labeled with 6-carboxy-4´,5´-dichloro-2´,7´-dimethoxy-fluorescein (JOE). All five loci are amplified simultaneously in a single tube and analyzed in a single injection.

The PowerPlex® S5 System is compatible with the ABI PRISM® 310, 3100 and 3100-Avant and Applied Biosystems 3130 and 3130xl Genetic Analyzers. The protocols presented in this manual were tested at Promega Corporation.
Amplification and detection instrumentation may vary. You may need to optimize protocols including cycle number and injection time (or loading volume) for each laboratory instrument. In-house validation should be performed.

The PowerPlex® S5 System provides all of the materials necessary for amplification of STR regions of purified genomic DNA.

The PowerPlex® S5 System provides all of the materials necessary for amplification of STR regions of purified genomic DNA.

Representative results of the PowerPlex® S5 System are shown in this figure
The PowerPlex® S5 System. A single DNA template (250pg) was amplified using the PowerPlex® S5 System. The amplification products were detected using an Applied Biosystems 3130xl Genetic Analyzer and a 3kv, 5-second injection. The results were analyzed using GeneMapper® ID software, version 3.2. Panel A. An electropherogram showing the peaks of the fluorescein-labeled loci: Amelogenin, D18S51 and D8S1179. Panel B. An electropherogram showing the peaks of the JOE-labeled loci: TH01 and FGA. Panel C. An electropherogram showing the 80bp to 300bp fragments of the Internal Lane Standard 600.

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