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SWAB SOLUTION

SwabSolution™ Kit, PunchSolution™ Kit and AmpSolution™ Reagent

 
 
Rapid Processing Solutions for PowerPlex® Systems
 

No matter your sample type, simplify your database or paternity workflow without diminishing STR profile quality.
SwabSolution™, PunchSolution™ and AmpSolution™ products streamline the processing of swabs and punches for PowerPlex® STR analysis without compromising your results.

 

While direct amplification from FTA® cards is transforming workflows in databasing and paternity testing labs, buccal swabs and non FTA cards are not ideal substrates for direct amplification. The Promega SwabSolution™ Kit, PunchSolution™ Kit and AmpSolution™ Reagent provide new, faster methods for processing swabs and card punches. Additionally, AmpSolution™ Reagent enables direct amplification from traditional PowerPlex® kits that were not originally intended for that application, minimizing the need to validate a new system to perform direct amplification.

   
  • Quickly and easily process fresh or old swabs with the SwabSolution™ Kit
  • Process nonFTA punches on the same plate with the PunchSolution™ Kit
    Perform direct amplification with FTA® punches using your validated PowerPlex® Systems including PowerPlex® 16 HS, ESI and ESX Systems with the AmpSolution™ Reagent

Swab Analysis

 

The SwabSolution™ Kit works in tandem with PowerPlex® Systems, including PowerPlex® 21, 18D, 16 HS, ESX, ESI, CS7 and Y23 Systems to enable reproducible, robust STR profiles from swab buccal samples.
As demonstrated in the "Buccal Swab Workflow", when using SwabSolution™ Kit, centrifugation or washes are no longer needed to extract DNA from the swab head. Eliminating those steps offers streamlined workflows and expanded throughput capabilities. Furthermore, quantification of DNA is possible, as is long-term storage of SwabSolution™ extracts for future use.

SwabSolution™ Kit—Buccal Swab Workflow.
   
Swab Sample, PowerPlex® 16 HS System
 
   
An OmniSwab™ buccal sample was pretreated with SwabSolution™ Reagent as recommended in the SwabSolution™ Kit Technical Manual TMD037. Amplification was performed using the PowerPlex® 16 HS System. Amplified products were separated on an Applied Biosystems 3130xl Genetic Analyzer (3kV, 5-second injection).

Non FTA Punch Analysis

PunchSolution™ Kit works in conjunction with PowerPlex® Systems, including the PowerPlex® 21, 18D, 16HS, ESX, ESI, CS7 and Y23 Systems to provide robust amplification profiles from nonFTA cards. As shown in the "NonFTA Punch Workflow", the PunchSolution™ Kit offers a simple sample preparation process to simplify analysis. At 10µl, the volume of PunchSolution™ Reagent allows easier punch handling than other pretreatment products that only use 2µl of solution. Higher quality, easier-to-interpret profiles are obtained, compared to other methods for processing nonFTA punches.

PunchSolution™ Kit—NonFTA Punch Workflow
   
Those samples treated with PunchSolution™ Reagent produce robust peaks, ensuring STR success, resultng in higher quality and easier to interpret profiles, when compared to other methods for non-FTA® punches.

Blood on S&S903, PowerPlex® 18D Amplification

One 1.2mm blood punch on S&S903 card was pretreated with PunchSolution™ Reagent as recommended in the PunchSolution™ Kit Technical Manual TMD038. Amplification was performed using the PowerPlex® 18D System. Amplified products were separated on an Applied Biosystems 3130xl Genetic Analyzer (3kV, 5-second injection).

FTA® Punch Analysis

 

5X AmpSolution™ Reagent enables direct amplification from FTA® punches with many traditional PowerPlex® Systems, including the PowerPlex® 16 HS, ESX, ESI and CS7 Systems

 
Buccal Samples on FTA® Punches, PowerPlex® ESX 17 System
Two 1.2mm punches from a buccal sample on an FTA® card were amplified using the PowerPlex® ESX 17 System. Prior to amplification, AmpSolution™ Reagent was added to the reaction mix. Amplified products were separated on an Applied Biosystems 3130xl Genetic Analyzer (3kV, 5-second injection).
 
   

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Municipio Libre No. 366 Col. Santa Cruz Atoyac C.P. 03310 Ciudad de México
Tels: 5601-3004 / 2856 / 2063
E-mail: jzogbi@dczogbi.com