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Collect, Isolate, Archive, and Transport
Nucleic Acids–All at Room Temperature!

FTA® Cards

• Capture nucleic acids rapidly in one easy step
• Nucleic acids are available for downstream applications in less than 30 minutes
• Store nucleic acids for years at room temperature
• FTA Cards are available in a variety of configurations to meet application equirements–Custom configurations are available on request

                               

Capture and Protect Nucleic Acids in One Easy Step

FTA® Cards utilize Whatman’s patented FTA Technology to simplify the handling and processing of nucleic acids. Simply apply your sample to the FTA Card. Cell membranes are lysed and nucleic acids are immobilized on the card for transport,
immediate processing or long-term room temperature storage. Captured nucleic acids are protected from nucleases, oxidation, and UV damage. FTA Cards inactivate organisms including blood
borne pathogens and prevent the growth of bacteria and other
microorganisms.

Store Nucleic Acids at Room Temperature for Years

Genomic DNA stored on FTA Cards at room temperature for over 11 years has been successfully amplified by PCR. No other product can make that claim.
FTA Cards offer a compact room temperature storage system that reduces the need for precious freezer space, improves sample accessibility and reduces storage costs.

Use with Virtually any Cell Type

The following is a partial list of the cell types that can be applied to FTA Cards

 

Captured Nucleic Acid is Ready for Downstream Applications in Less than 30 Minutes

Captured nucleic acids are ready for purification when you are. Just take a sample disc from the FTA Card, wash with FTA
Purification Reagent and rinse with TE-1 buffer. The washed disc is ready to use in applications such as PCR, RFLP analysis, and RT-PCR.
A Protocol for Elution of DNA from FTA Cards for human identification studies, i.e., identification of CODIS loci, is available.
Contact Technical Services for more information.

             
  Apply specimen and
allow to dry completely.
             
             
Disc Removal:
Punch a disc out of the
sample area on the FTA Card.
FTA Reagent Washes:
Place the disc in a tube
and wash three times.
           
TE-1 Rinses:
Rinse disc two times.
FTA Cards are available in either white or pink (Indicating) formats.
White FTA Cards are recommended for blood samples, plant tissues, and other easily identified samples.
Indicating FTA Cards are pink and turn white upon sample addition. Indicating FTA Cards are recommended for buccal cells, cultured cells and other clear samples.
           
Allow the disc to dry completely.
           
               
Direct to PCR:
Add PCR master mix
directly to the disc in tube.
               
FTA Cards are available in 1, 2, 3 and 4 part configurations.
Custom configurations are available upon request.
Call 1-866-PureDNA for details.

FTA Purification Reagent
Removes heme, PCR inhibitors, and other potential contaminants to ensure superior quality DNA for downstream analysis.

FTA Gene Card Tray
Holds two FTA Gene Cards for use in automatic liquid handling systems.

Sterile Foam Tipped Applicator
Easy-to-use applicator for the noninvasive collection and transfer of buccal cells to FTA Cards.

FTA® Classic Cards
Four sample areas for storage of up to 500µL whole blood
or 100µL plant homogenate per card. Convenient for multiple
applications of the same specimen or collection of multiple animal or plant samples. Also available in Indicating (pink) FTA format.
FTA Mini Card
Two sample areas for storage of up to 250µL whole blood or
50µL plant homogenate per card. Convenient for protocols that
require different locations for testing and archiving samples.
Also available in Indicating (pink) FTA format.
FTA Micro Card
One sample area for storage of up to 125µL whole blood or 25µL
plant homogenate per card. Recommended when only one
sample is needed. Also available in Indicating (pink) FTA format.
FTA Gene Card
Three sample areas in a chipboard frame for storage of up to
225µL whole blood or 30µL plant homogenate per card.
Can be run in most automatic dispensing/pipetting systems
when used with the FTA Gene Card Tray.
   

Harris Micro Punches, Disposable Uni-Core Punches and Cutting Mat

For the precise sample disc removal from FTA Cards. The 1.2mm Punches are recommended for use with whole blood and samples with high DNA content. The 2.0mm Punches are recommended for use with buccal cells, plasmids, and samples with lower DNA content.

Multi-Barrier Pouches
For transporting or storing FTA Cards. Protects cards from environmental contamination. Tamper-evident seal maintains sample security for forensics samples. A resealable pouch is also available when multiple access to FTA cards is needed.

FTA Card Mailer
A rigid protective cardboard mailer for transporting FTA Cards without biohazard labeling.

Storage Desiccant Packets
Ensure that FTA Cards remain dry during transport or storage. Contains indicator that changes color to verify moisture adsorption.

CloneSaver™ Cards
     
FTA® Cards and Indicating FTA Cards*  
*Indicating FTA Cards change color from pink to white when sample is applied and are recommended for use with clear samples.
FTA Gene Cards are compatible with automated liquid sampling systems when used with FTA Gene Card Trays.
Custom configurations of all FTA Cards are available upon request.
 
 
Reagent and FTA Accessories